The Biomolecular NMR Facility houses three spectrometers, two of which operate at 600 MHz (14 Tesla), and one at 500 MHz (11.7 Tesla). In the summer of 2012, one 600 MHz and the 500 MHz spectrometer were upgraded to Bruker AVANCE III NMR  consoles. The second 600 MHz instrument is a Varian INOVA spectrometer equipped with an ultra-sensitive cold probe. The instruments are all optimized for acquisition of large data sets necessitated by multidimensional NMR of isotopically labeled macromolecules. They are not capable of imaging or performing experiments on solids. Signup must be requested in advance. 

      All instruments share the following features:
    • Four RF channels (i.e., capable of 1H / 13C / 15N / 2H experiments)
    • Waveform generators on three RF channels (1H, 13C, 15N is our standard configuration)
    • 50 W 1H amplifiers and 300 W decoupling amplifiers
    • Oversampling with digital filtering and quadrature
    • Temperature control ranging from -20 C to +150 °C using dry compressed air (0 - 50°C on the cold probe)
    • A complete suite of  biomolecular NMR experiments, updated regularly
    • One spectrometer equipped with a SampleXpress sample changing robot.
    The spectrometers currently have the following equipment:
    Spectrometer Probes & Distinguishing Features
    600-2 (south)
    • Varian Unity Spectrometer
    • 5 mm Cold Probe, triple (1H {13C, 15N}) PFG (z-axis)
    • Variable temperature from 0 - 50˚C
    600-1 (north)
    • Bruker AVANCE III HD Console
    • 5 mm PFG (z-axis) triple probe (1H {13C, 15N}) 
      • 5 mm TXI probe
    • SampleXpress sample changing robot. Can handle up to 60 samples.
    • Automatic tuning and matching
    • Variable temperature range from -20 - +150˚C
    500
    • Bruker AVANCE III Console
    • 5 mm PFG (z-axis) triple probe (1H {13C, 15N})
      • 5 mm TXI probe
    • Automatic Tuning and matching
    • Variable temperature range from -20 - +150˚C

    Other Equipment

    • Gilson 215 sample preparation system for 5 mm NMR tubes
      • Provides robotic sample loading.